Dynamic Biosensors 10X Buffer HE140 pH 7.4
Quick guide for preparing and storing the Dynamic Biosensors 10X Buffer HE140 pH 7.4. Includes dilution instructions, composition details, and storage requirements for research use.
Table of contents
Important Information
The Dynamic Biosensors 10X Buffer HE140 pH 7.4 is intended for research use only. Please check the expiry date on the product label before use, as the product has a limited shelf life.
Product Description
The 10X Buffer HE140 pH 7.4 is a 0.2 µm sterile-filtered solution. Its composition includes:
- 100 mM HEPES
- 1.4 M NaCl
- 500 µM EDTA
- 500 µM EGTA
- 0.5% Tween20
Preparation
To prepare the buffer for use, follow these steps:
- Take 50 mL of the complete 10X Buffer HE140 pH 7.4 solution.
- Mix with 450 mL of ultrapure water.
- The resulting buffer will contain 10 mM HEPES, 140 mM NaCl, 50 µM EDTA, 50 µM EGTA, and 0.05% Tween20.
Storage: The diluted buffer should be stored at 2-8°C.
Contact Information
For order information or technical support, contact Dynamic Biosensors GmbH or Dynamic Biosensors, Inc. via email at [email protected] or [email protected].
Practical help
Common problems
Check the expiry date on the label; do not use if expired.
Ensure the 10X solution is diluted with 450 mL of ultrapure water to achieve the correct working concentration.
Before use
- Verify the expiry date on the label
- Prepare 450 mL of ultrapure water
- Ensure storage conditions are 2-8°C
Manual page author
David Miller
Documentation analyst
Organizes user manual content into clear summaries, with attention to model details, product context, and everyday usability.